The forward on Tuesday suffered a pelvic contusion while scoring the winner against Iran but was posting on social media from his hospital bed later that evening, allaying fears he would be ruled out of the knockout-stage game.
Weston McKennie was nursing a leg injury but starts, while Josh Sargent is a more pressing concern after damaging an ankle and misses out.
For Netherlands, head coach Louis van Gaal has a fully fit squad apart from back-up right-back Jeremie Frimpng, who has picked up an ankle injury in training.
The concern for the veteran boss will be Nathan Ake, who is one yellow card away from missing a possible quarter-final.
Cody Gakpo is looking to keep up his record of scoring in every World Cup 2022 game so far.
Starting Netherlands XI: Noppert; Timber, Van Dijk, Ake; Dumfries, De Roon, F. De Jong, Blind; Klaassen; Gakpo, Depay
Starting USA XI: Turner; Dest, Zimmerman, Ream, Robinson; McKennie, Adams, Musah; Weah, Ferrira, Pulisic