Iran finished third with three points. The team has failed to advance in all six of its World Cup appearances.
"The dream is over with this result," Iran coach Carlos Queiroz said. "The US started the game much better than us, more quick with better control of the game."
There was heightened tension surrounding the match because of the strained relations between the United States and Iran's Islamic government. Iran had famously upset the US 2-1 in the 1998 tournament in France, eliminating the Americans.
Pregame protocols were normal, unlike the 1998 game, when Iranians presented the Americans with white flowers and the teams posed for a joint photo.
Raucous Iranian fans with horns and drums in the crowd of 42,127 filled the lower bowl behind one goal at Al Thumama Stadium and were far louder than the red-white-and-blue US supporters at the other end and behind the American bench.
Iran World Cup 2022 squad
Goalkeepers: Alireza Beiranvand (Persepolis), Amir Abedzadeh (Ponferradina), Seyed Hossein Hosseini (Esteghlal), Payam Niazmand (Sepahan)
Defenders: Ehsan Hajsafi (AEK Athens), Morteza Pouraliganji (Persepolis), Ramin Rezaeian (Sepahan), Milad Mohammadi (AEK Athens), Hossein Kanaanizadegan (Al Ahli), Shojae Khalilzadeh (Al Ahli), Sadegh Moharrami (Dinamo Zagreb), Rouzbeh Cheshmi (Esteghlal), Majid Hosseini (Kayserispor), Abolfazl Jalali (Esteghlal)
Midfielders: Ahmad Noorollahi (Shabab Al Ahli), Saman Ghoddos (Brentford), Vahid Amiri (Persepolis), Saeid Ezatolahi (Vejle), Alireza Jahanbakhsh (Feyenoord), Mehdi Torabi (Persepolis), Ali Gholizadeh (Charleroi), Ali Karimi (Kayserispor)
Forwards: Karim Ansarifard (Omonia Nicosia), Sardar Azmoun (Bayer Leverkusen), Mehdi Taremi (Porto)
Who are the star names in the squad?
Iran’s main star in Qatar can still be Mehdi Taremi, who scored 20 league goals last season to fire Porto to the Portuguese title – and netted twice in defeat against England in their opener in Qatar.
Other names to look out for are Bayer Leverkusen forward Sardar Azmoun, and Alireza Jahanbaksh, the captain, who once scored with an overhead kick for Brighton against Chelsea.
Why did Iran's players refuse to sign their national anthem?
Iran’s footballers won admirers around the world for courageously taking a stand against the oppressive regime in Tehran despite the risk of personal reprisals.
Before the start of their World Cup game against England, Iran’s players refused to sing their country’s national anthem, while their captain spoke up in support of the anti-government protests that have resulted in more than 300 deaths so far.
Emboldened by the leadership of their team, supporters in the stadium also voiced their dissent by jeering the national anthem and holding up placards demanding greater freedoms for women.
They included female football fans who were attending a football match for the first time in their lives because they are not allowed to do so in their home country.
However, the Iran team did sing the country's national anthem before their match against Wales on Friday, amid reports that protesters had been harassed by government-supporting fans outside of the stadium.
Some of Iran's players appeared to be mumbling the song quietly while fans booed and jeered from the seats behind them. Several supporters were seen crying and protesters with signs were confronted by security guards.
Iran has been engulfed by mass demonstrations after 22-year-old woman Mahsa Amini died in custody while she was being detained by the so-called morality police for breaking the country’s strict Islamic dress code.
As well as hundreds of deaths, the protests have resulted in thousands of arrests in a country that uses torture and arbitrary detention to crush dissent. There were also calls for Iran to be thrown out of the World Cup tournament.
The Iranian team had, until their first fixture, remained vehemently apolitical but the players bravely decided to use the platform given to them by the World Cup to embarrass Tehran on an international stage.
What are Iran’s fixtures?
What is Iran’s World Cup record?
Iran are beginning to establish themselves as World Cup regulars, with the 2022 World Cup in Qatar being the third consecutive tournament for which they have qualified.
They have appeared in six World Cup finals in total (1978, 1998, 2006, 2014, 2018 and 2022), but have never reached the knockout stages. Their 1-0 win over Morocco at the Russia World Cup in 2018 was only their second win in the tournament.
Their results in recent times have not gone according to what Iran’s performances merited, however. The four points they got in 2018 (which included a draw against Portugal and a narrow loss against Spain) would usually have been enough to progress from the group stages. In 2014 it took a late moment of magic from Lionel Messi to stop Iran holding finalists Argentina to a goalless draw.
Argentina | Australia | Belgium | Brazil | Cameroon | Canada | Costa Rica | Croatia | Denmark | Ecuador | England | France | Germany | Ghana | Iran | Japan | Mexico | Morocco | Netherlands | Poland | Portugal | Qatar | Saudi Arabia | Senegal | Serbia | South Korea | Spain | Switzerland | Tunisia | Uruguay | USA | Wales